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A total of forty-nine blood samples and twenty-eight blood smears received for diagnosis of haemorrhagic septicaemia (I-IS) were processed for detection of Pasteurella multocida DNA by polymerase chain reaction (PGR), using species specific {PM-PCR} and type-B specific (HS-B PCR) primers. Culturing of the forty-nine blood samples yielded only five isolates of P. multocida. Thirteen samples (eight blood samples and five blood smears) found to be positive by PMPCR, generated an amplified product of 460 bp. The entire samples tested positive by PMPCR were confirmed as type•B Y multocida by HS-B specific PCR, which generated an isolates-DP1 & DP2j of p. multocida, which were used for comparison in multiplex PCR gave only single band corresponding to 460 by (Figs. 5, 6). Only serotype B P. multocida generated two bands in multiplex PCR. These results are in accordance with those of Townsend et al. (2000) and Dutta et al. {2004).