GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
oleh: Jack Tuffin, Musleeha Chesor, Valeryia Kuzmuk, Tim Johnson, Simon C. Satchell, Gavin I. Welsh, Moin A. Saleem
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-12-01 |
Deskripsi
Tuffin et al. demonstrate a method for culturing conditionally immortalized human podocytes and GEnCs in 3D by forming spheroid cultures termed GlomSpheres, which are found to segregate in an organized manner. These are shown to establish a GBM-like extracellular matrix with increased cell differentiation and maturity when compared to 2D cultures and their use demonstrated in pharmaceutical screening using rapid imaging approaches.