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Objective: to estimate the magnitude of the cardioprotective properties of sevoflurane and propofol, when they are used alone and in combination in the acute experiment on rats after total ischemia/reperfusion (I/R). Materials and methods. The investigation was conducted on 50 male albino outbred rats weighing 202±19 g (M±ff), by using a total I/R model. The animals were randomized into 5 groups: 1) intraperitoneal chloral hydrate 300 mg/kg; 15-minute mechanical ventilation (MV); 2) inhalation induction with sevoflurane in an exsiccator; MV; injection of about 2—2.5 MAC sevoflurane in the expiratory breathing circuit for 15 minutes — anesthetic preconditioning simulation (APS); 3) anesthesia and APS with sevoflurane; then 10-minute ischemia simulation, followed by resuscitation and 5-minute reperfusion; 4) chloral hydrate anesthesia; injection of propofol 2.5 mg/kg in the right internal jugular vein; APS simulation; 5) chloral hydrate anesthesia; injection of propofol 2.5 mg/kg in the right internal jugular vein; and simulation of APS with sevoflurane. The hearts were extracted from all the animals and homogenized for further examination. The concentrations of total and phosphorylated glycogen synthase kinase-3/8 (GSK-3/8 and pGSK-3/8, respectively) were estimated by Western blotting. Results. The concentration of total GSK-3/8 was comparable in the groups. Group 2 showed a 1.5-fold increase in pGSK-3^ concentrations  (p<0.05), a more increment (by more than twice) was observed in Group 3 (p<0.05). The concentration of pGSK-30 rose slightly in Group 4. In Group 5, the level of pGSK-30 decreased approximately twice that in Group 1 (p<0.05). Conclusion. Sevoflurane can be expected to have cardioprotective properties manifested as a considerably elevated p-GSK-Эв level. Propofol fails to exert a significant cardioprotective effect and when it is used in combination with sevoflurane, the latter is likely to completely deprive of the cardioprotective properties, by lowering the level of p-GSK-3^ below the reference values, which may be associated with the antioxidant properties of propofol. Evidently, further investigation should be continued to form ischemia/reperfusion groups when these anesthetics are co-administered. Key words: anesthetic preconditioning, sevorane, glycogen synthase kinase-3/3, Western blotting.