Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
oleh: Vivek K. Mutalik, Pavel S. Novichkov, Morgan N. Price, Trenton K. Owens, Mark Callaghan, Sean Carim, Adam M. Deutschbauer, Adam P. Arkin
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-01-01 |
Deskripsi
Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.