Differences in RNA polymerase II complexes and their interactions with surrounding chromatin on human and cytomegalovirus genomes
oleh: Benjamin M. Spector, Mrutyunjaya Parida, Ming Li, Christopher B. Ball, Jeffery L. Meier, Donal S. Luse, David H. Price
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-04-01 |
Deskripsi
Here the authors digested chromatin with DNA fragmentation factor (DFF) prior to chromatin immunoprecipitation (DFF-ChIP) to depict transcription complex interactions with neighboring nucleosomes in cells. Applying this method to human cytomegalovirus (HMCV)-infected cells, they find that the viral genome is underchromatinized, leading to fewer transcription complex interactions with nucleosomes.