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As one of the important ways to prepare radioisotopes, the role and status of accelerator produced nuclides have become increasingly prominent after entering the new century. In the present paper, a brief overview on development status of radioisotope preparation technology by accelerators at home and abroad was performed. In particular, the research progress of 18F, 64Cu, 68Ge/68Ga, 89Zr, 111In, 211At, 225Ac and other important medical radioisotope preparation technologies are introduced. The application prospects of isotopes prepared by domestic accelerators are also prospected.