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Nanobiotechnology has gained attention in the field of medicine and can provide solutions to many questions of medicine including antibiotic resistance. This study was performed to assess the antibacterial activity of iron nanoparticles synthesized by green synthesis, using Syzgium aromaticum (clove) as a reducing and stabilizing agent for an anticipatory use to address antibiotic resistance. Antibacterial activities of iron nanoparticles (FeNPs) analyzed against Gram-positive (Bacillus subtilis, Enterococcus faecalis) and Gram-negative (Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa) bacterial species anticipated the use of earlier to serve the purpose.  FeNPs exhibited the highest antibacterial potential against Enterococcus faecalis. Further studies should be performed to know the exact potential of FeNPs against these bacterial strains.