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<i>Gardnerella vaginalis</i> (<i>G. vaginalis</i>) is the major bacteria detected in women with bacterial vaginosis (BV). <i>Prevotella bivia</i> (<i>P. bivia</i>) has been demonstrated to show a symbiotic relationship with <i>G. vaginalis</i>. Some men have been shown to be colonized with <i>G. vaginalis</i> in their urogenital or anorectal tracts, however genitourinary infections in males, including balanitis and urethritis, due to this organism appear to be much less common. In this report, we summarize previous cases of men with <i>G. vaginalis</i> infection, and we present a rare and unusual case of a unilateral scrotal abscess caused <i>by G. vaginalis</i> in co-infection with <i>P. bivia</i>.