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In Vitro Digestion of Microcapsule Carriers for Oral Delivery of Bioactive Compounds for Diabetes Treatment and Their Inhibitory Effect on the DPP-4 Enzyme
oleh: Edwin García-Miguel, Veera C.S.R. Chittepu, Poonam Kalhotra, José Proal-Nájera, Guillermo Osorio-Revilla, Tzayhrí Gallardo-Velázquez
Format: | Article |
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Diterbitkan: | MDPI AG 2019-11-01 |
Deskripsi
Empty microcapsules, originally designed as carriers of bioactive peptides, were prepared by the combined method of a double-emulsion complex with coacervation spray drying and were subjected to an in-vitro digestion process, producing peptides from the whey protein contained in the microcapsule walls. The inhibitory effect of the enzyme dipeptidyl peptidase-4 (DPP-4) and modulation of the insulin receptor of hydrolyzed microcapsules were evaluated. The hydrolysate of the microcapsules was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, showing the presence of low-molecular-weight peptidic compounds, which apparently were responsible for the DPP-4 inhibitory effect. Fluorescence analysis showed that the effect of the hydrolyzed microcapsules on the insulin receptor was 40% that of insulin. The inhibition of DPP-4 was 54.7%. This work demonstrated that empty microcapsules initially designed as carriers of functional peptides also have the capability to inhibit DPP-4 and modulate insulin receptors by themselves.