Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
oleh: Digvijay Singh, Samuel H. Sternberg, Jingyi Fei, Jennifer A. Doudna, Taekjip Ha
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-09-01 |
Deskripsi
CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.