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Rice (Oryza sativa) is the predominant staple food crop belonging to the Poaceae family. In this study, complete chloroplast genome sequence of O. sativa aus-type variety Nagina-22 was characterized through de novo assembly. The genome is a circular DNA molecule of 134,503 bp and has typical quadripartite structures including large single copy region (80,548 bp), small single copy region (12,347 bp), and a pair of inverted repeats (20,804 bp). A total of 120 genes were predicted in the genome, including 77 protein-coding genes, 8 open reading frame genes, 31 tRNA genes, and 4 rRNA genes. Phylogenetic analysis confirmed a close taxonomical relationship with O. sativa ssp. Indica species.