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Results are presented regarding the development of recycled paper based impedance biosensors with screen printed interdigitated electrode structures (IDES). The sensors show a response to increasing salt concentrations in the range 30–100 mM NaCl. To prove the feasibility of using recycled paper, biofunctionalization with a glucose sensitive enzyme mixture was performed by inkjet printing. The quantification of the glucose sensitive colour change reaction in paper was investigated and a trend is found in the range of 6–90 mg/dL. Subsequently, measurements with a wireless electronic readout system were performed on an electrochemical assay showing a decrease of the normalized sensor response dependent from the glucose concentration in the range 0–80 mg/dL.