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The enteropathogenic <i>Yersinia</i> genus is commonly detected in wildlife including wild boars. Difficulties in its cultivation may hamper subsequent epidemiological studies and outbreak investigations. Multiple-locus variable-number tandem repeat analysis (MLVA) of <i>Yersinia (Y.) enterocolitica</i> and <i>Y. pseudotuberculosis</i> has proven useful in source attribution and epidemiological studies but has hitherto relied on the analysis of isolates. In the present study, MLVA profiles generated from 254 isolates of <i>Y. enterocolitica</i> indicated similarities between human, pig and rodent isolates. Further, MLVA analyses of 13 <i>Y. pseudotuberculosis</i> pure-cultured isolates were compared to MLVA analyses performed directly on the 14 PCR-positive enrichment broths from which the isolates originated, which showed matching MLVA profiles. This indicates that MLVA analysis performed directly on enrichment broths could be a useful method for molecular epidemiological investigations. In addition, 10 out of 32 samples of wild boar minced meat obtained from private hunters and from approved wild-game-handling establishments were PCR-positive for the presence of <i>Y. enterocolitica</i> and may indicate a risk for public health.