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Bovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of <i>Leptospira interrogans</i> serogroup Pomona that occurred in cattle farms within the Nebrodi Park and was mainly characterized by full-term abortion. Blood and urine samples were collected at different time points from animals of six different farms (Farms A–F) sharing the same grazing area. Research of antibodies against pathogenic <i>Leptospira</i> species in serum samples was carried out via Micro Agglutination Test (MAT). Urine samples were subjected to pathogen isolation and molecular analyses via TaqMan Real Time-PCR. Genotyping of <i>Leptospira</i> species was obtained by Multi-locus sequence typing. MAT detected antibodies against <i>Leptospira interrogans</i> serogroup Pomona in serum samples of all the farms. Pathogenic <i>Leptospira</i> spp. DNA and culture isolation was obtained from urine samples. Genotyping confirmed the excretion of <i>L. interrogans</i> serogroup Pomona. This study describes clinical manifestations, diagnostic implications and epidemiological characteristics of an outbreak in cattle due to <i>L. interrogans</i> Pomona in a protected multi-host area, where domestic and wild animals share the same habitat, suggesting a role of wild species in transmission and persistence of Pomona serogroup among cattle.