Pooled library screening with multiplexed Cpf1 library
oleh: Jintan Liu, Sanjana Srinivasan, Chieh-Yuan Li, I-Lin Ho, Johnathon Rose, MennatAllah Shaheen, Gang Wang, Wantong Yao, Angela Deem, Chris Bristow, Traver Hart, Giulio Draetta
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-07-01 |
Deskripsi
AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.