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Chlorophyll-a (Chl-a) concentration is one of the important indicators in water bodies for assessing the ecological health of water quality. In this paper, an OGolden-DBO-XGBoost Chl-a concentration inversion model is proposed using Wuliangsu Lake as the study area, and by combining the Sentinel-2 remote-sensing satellite images and measured Chl-a concentration data in Wuliangsu Lake, the XGBoost model is optimized using the hybrid-strategy-improved dung beetle optimization algorithm (OGolden-DBO), and an OGolden-DBO-XGBoost Chl-a concentration inversion model. The OGolden-DBO-XGBoost model’s coefficients of determination (R²s) were 0.8936 and 0.8850 on the training set and test set, according to the results. The root mean squared errors (RMSEs) were 3.1353 and 2.9659 μg/L, and the mean absolute errors (MAEs) were 1.8918 and 2.4282 μg/L. The model performed well and provided a strong support for the detection of Chl-a concentration in Wuliangsu Lake.