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This study aimed to investigate the diet composition, potential food preferences, and trophodynamics (trophic level) of Riverine catfish Rita rita collected from the Surma River, Bangladesh. For this purpose, gut contents of 151 of R. rita were examined. Gut content analysis revealed that fish fed frequently on mollusks (26.36%) and arthropods (17.78%) followed by plant matters (16.96%), insects (12.75%), teleosts (12.52%), and rice seeds (6.02%). The mean trophic level was 2.411 ± 0.251, indicating that the studied fish are largely carnivorous. The highest mean trophic level was observed in February (2.794 ± 0.207), which is significantly higher than other months (P < 0.05). Moreover, diets were not significantly different among size groups. Overall, gut content and trophic level analyses revealed that R. rita is largely a carnivorous species that occupies the intermediate level in the trophic pyramid.