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<i>Neofusicoccum parvum</i> is a fungal plant-pathogen belonging to the family <i>Botryosphaeriaceae</i>, and is considered one of the most aggressive causal agents of the grapevine trunk disease (GTD) Botryosphaeria dieback. In this study, the mycovirome of a single strain of <i>N. parvum</i> (COLB) was characterized by high throughput sequencing analysis of total RNA and subsequent bioinformatic analyses. Contig annotations, genome completions, and phylogenetic analyses allowed us to describe six novel mycoviruses belonging to four different viral families. The virome is composed of two victoriviruses in the family <i>Totiviridae</i>, one alphaendornavirus in the family <i>Endornaviridae</i>, two mitoviruses in the family <i>Mitoviridae</i>, and one narnavirus belonging to the family <i>Narnaviridae.</i> The presence of the co-infecting viruses was confirmed by sequencing the RT-PCR products generated from total nucleic acids extracted from COLB. This study shows that the mycovirome of a single <i>N. parvum</i> strain is highly diverse and distinct from that previously described in <i>N. parvum</i> strains isolated from grapevines.