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This paper develops a strawberry robot-arm based on computer vision, and uses deep learning to localize targets for strawberry robot-arm. In order to speed up localization, the algorithm segments the image from strawberry robot-arm based on color prior knowledge to get strawberry proposals, then inputs proposals to convolutional neural network(CNN) pretrained and gets the probability for every proposal, and finally chooses the target with the highest probability as the localization result. Experiment results show that this algorithm can localize target in real time and with high precision.