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Aim:It was aimed to determine the differences in time of physeal closure and the appearance of humeral distal epiphyses in the prepubertal period between genders.Methods:Radiological data of the first 200 healthy children in the prepubertal period, who underwent diagnostic X-ray imaging of the elbow due to the complaints of the elbow region between January 2012 and January 2017, were collected. The presence of lateral epicondyle, lateral condyle, trochlea, and medial epicondyle epiphysis cores on the elbow radiographs was classified and labeled by age and gender. The ages, in which the lines between lateral epicondyle and lateral condyle, lateral condyle and trochlea, medial epicondyle and humerus, humerus and lateral epicondyle, and lateral epicondyle and trochlea epiphyses were found to be closed, were documented by gender.Results:Examining the mean ossification time by gender for lateral epicondyle, trochlea, medial epicondyle, and lateral condyle, this time was found to be nine months early, nine months early, 10 months early, and two months late for the girls, respectively. The mean physeal closure time in girls were found to be 13 months earlier for the lateral epicondyle-lateral condyle physis, seven months earlier for lateral condyle-trochlea physis, eight months later for lateral epicondyle-lateral condyle-trochlea-humerus physis, and nine months later for medial epicondyle-humerus physis.Conclusion:In the literature, a delay was observed in ossification time of lateral condyle in girls and medial epicondyle in both genders. When compared with girls, the medial epicondyle and fusion times were found to be earlier in boys than in girls.