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This paper presents results of a malacological survey of the Oslava River, a tributary of the Jihlava River (Czech Republic, Southeastern Moravia). Sixteen species of aquatic molluscs (8 gastropods, 8 bivalves) were found at 14 sites during the research conducted from 2010 to 2018. The research was focused on the endangered bivalve Unio crassus. Its population was confirmed at the lower river section and is connected with the population in the Jihlava River.