Simultaneous orientation and 3D localization microscopy with a Vortex point spread function
oleh: Christiaan N. Hulleman, Rasmus Ø. Thorsen, Eugene Kim, Cees Dekker, Sjoerd Stallinga, Bernd Rieger
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-10-01 |
Deskripsi
Molecular orientation is often ignored during single-molecule localisation microscopy. Here, the authors use a Vortex point spread function in order to simultaneously estimate the 3D position, dipole orientation and degree of rotational constraint, within 30% of the Cramér-Rao bound limit.