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<i>Staphylococcus aureus</i> phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43,513 bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage Henu2 belongs to the family <i>Siphoviridae</i> and possesses an isometric head (63 nm in diameter). The latent time and burst size of Henu2 were approximately 20 min and 7.8 plaque forming unit (PFU)/infected cells. The Henu2 maintained infectivity over a wide range of temperature (10–60 °C) and pH values (4–12). Phylogenetic and comparative genomic analyses indicate that <i>Staphylococcus aureus</i> phage Henu2 should be a new member of the family of <i>Siphoviridae</i> class-II. In this paper, Phage Henu2 alone exhibited weak inhibitory activity on the growth of <i>S. aureus</i>. However, the combination of phage Henu2 and some antibiotics or oxides could effectively inhibit the growth of <i>S. aureus</i>, with a decrease of more than three logs within 24 h in vitro. These results provide useful information that phage Henu2 can be combined with antibiotics to increase the production of phage Henu2 and thus enhance the efficacy of bacterial killing.