Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
oleh: Martina Pavletić, Marija Mazor, Mate Lerga, Tatjana Mileta, Jelena Železnjak, Tina Ružić, Sanda Ravlić, Dora Palčevski, Jelena Kirinčić, Silvestar Mežnarić, Ana Mišković, Maja Materljan, Alan Šustić, Berislav Lisnić, Vanda Juranić Lisnić
| Format: | Article |
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| Diterbitkan: | MDPI AG 2021-12-01 |
Deskripsi
During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.