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Nocardia spp are aerobic, Gram positive, weakly acid fast and non-motile bacteria. The bacteria invade the human body from the environment via trauma or the respiratory tract and cause cutaneous, pulmonary, and systemic disorder. So far, number of isolation techniques are described including: 1. paraffin baiting technique, 2. humic acid-vitamin agar, and 3. selective buffered charcoal-yeast extract medium e. g.