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<b>Aim:</b> The aim of the study was to conduct the in vitro antibiotic susceptibility test of Escherichia coli isolated from the migratory birds available in winter season at Hakaluki Haor (lagoon) of Bangladesh<p> <b>Materials and Methods:</b> A total of 100 Escherichia coli strains were isolated and subjected for in vitro antibiotic susceptibility test. Colistin sulphate, gentamicin, azithromycin, levofloxacin, ciprofloxacin, tetracycline, amoxicillin and metronidazole were used to detect the antibiotic susceptibility pattern of those isolates. The antibiogram status of each antibiotic was determined by measuring the diameter of the zone of inhibition due to the diffusion of the agent into the medium surrounding the disc.<p> <b>Results:</b> A high of 96%, 89% and 88% Escherichia coli isolates showed the sensitivity to colistin sulphate, levofloxacin and ciprofloxacin, respectively, followed by azithromycin (79%), gentamicin (77%), amoxicillin (63%) and tetracycline (46%). No isolate was sensitive to metronidazole.<p> <b>Conclusion:</b> Colistin sulphate, levofloxacin and ciprofloxacin have the potential effects against Escherichia coli in migratory birds and further study should be performed to evaluate the cross linkage of this Escherichia coli isolate with that of found in other poultry and human.