Immunofluorescence (Indirect Staining) Protocol for Adherent Cells
oleh: Ran Chen
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2012-02-01 |
Deskripsi
In this protocol, different types of adherent cells are fixed to coverslips by 4% paraformaldehyde. Target proteins are then stained by specific antibodies and fluorescent 2nd antibodies. This protocol therefore provides a method for immunostaining of adherent cells.