A type I MADS-box gene is differentially expressed in wheat in response to infection by the stripe rust fungus
oleh: J. Guo, X. -X. Shi, J. -S. Zhang, Y. -H. Duan, P. -F. Bai, X. -N. Guan, Z. -S. Kang
| Format: | Article |
|---|---|
| Diterbitkan: | Institute of Experimental Botany of the Czech Academy of Sciences 2013-09-01 |
Deskripsi
The gene, designated TaMADS2, was obtained from wheat leaves infected with the wheat stripe rust fungus by in silico cloning and RT-PCR. TaMADS2 encodes a predicted 159-amino-acid polypeptide that contains a highly conserved MADS domain. Phylogenetic analysis revealed that TaMADS2 is a type I MADS-box gene. The TaMADS2 transcript was detected in wheat leaves, stems, and roots. The expression of TaMADS2 was substantially down-regulated in the compatible interaction between wheat and Puccinia striiformis f. sp. tritici (Pst) at 36 and 48 h post-inoculation (hpi), whereas in the incompatible interaction the down-regulation was only observed at 48 hpi. Exogenous salicylic acid (SA) and abscisic acid (ABA) greatly induced the expression of TaMADS2 at 12 h post treatment (hpt), whereas methyl jasmonate (MeJA) down-regulated TaMADS2 at 6 hpt by approximately two-fold.