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reast cancer, the most frequent cancer in females is the major cause of morbidity and mortality in the world as well as in Pakistan. Screening of breast cancer or early detection requires a reliable biomarker that has high specificity and sensitivity for use in clinical practice. Certain diagnostic biomarker have been identified but are not fit for clinical use due to their low sensitivity like MUC1 (mucin glycoprotein) and CA 15-3 and CA 27-29 (cancer antigens)…