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We have characterized the complete genome sequence of an Australian isolate of banana streak CA virus (BSCAV). A greater-than-full-length, cloned copy of the virus genome was assembled and agroinoculated into five tissue-cultured plants of nine different <i>Musa acuminata</i> banana accessions. BSCAV was highly infectious in all nine accessions. All five inoculated plants from eight accessions developed symptoms by 28 weeks post-inoculation, while all five plants of <i>M. acuminata</i> AA subsp. <i>zebrina</i> remained symptomless. Symptoms were mild in six accessions but were severe in Khae Phrae (<i>M</i>. <i>acuminata</i> subsp. <i>siamea</i>) and the East African Highland banana accession Igisahira Gisanzwe. This is the first full-length BSCAV genome sequence reported from Australia and the first report of the infectivity of an infectious clone of banana streak virus.