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<i>Candida albicans</i> is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting <i>C. albicans</i> with the use of electrochemical sensors. Through this experiment, we propose a method for the detection of <i>C. albicans</i> in the system through the use of personal glucose meters. The hemicellulase was used to break down the cell wall of <i>C. albicans</i> to glucose and oligo, which can be detected by a glucose meter. The spiked samples were prepared suspending <i>C. albicans</i> in urine and serum, demonstrating the feasibility of the developed method in a real situation.