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Mitogen-activated protein kinase cascades are one of the many systems that allow plants to survive and defend themselves against pathogens and other environmental stresses. Numerous scientific investigations rendered insights to molecular signaling pathways that take place in an event of a stress such as soil salinity. Despite the known functions and locations of proteins that play a role in these pathways, very little is known about upstream protein partners. In this paper, we elucidate biological functions and molecular locations of Arabidopsis thaliana MKK1 protein through data mining predominantly from STRING and BAR databases. Results revealed AtMEKK1 and CRLK1 as upstream protein partners. In addition, AtMKK2 was further analyzed as a redundant protein to AtMKK1.