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Density profile of three types of medium density fiberboard (MDF) panels used in Iran market was studied through direct method and gradually removing of layer. Modulus of elasticity, bending strength, internal bond, water absorption and thickness swelling were also determined. The results show that the shape of density profiles in all types of panels is nearly symmetric and normal, but the profil density in one was deeper, so it showed higher modulus elasticity and bending strength. No significant difference in internal bond was observed between different types of panels.