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Six <i>N</i>-heterocyclic Carbene based Ionic Liquids (ILs) have been synthesized by conventional methods. The ILs were spectroscopically characterized by NMR and FT-IR techniques. Their <i>in vitro</i> antimicrobial activities were determined towards gram-positive and gram-negative bacterias and yeast strains using minimum inhibition concentration (MIC) assay. The best inhibition performances were obtained with compound <b>1</b> due to its more hydrophilic nature compared with the others. It exhibited 1 mg L^–1 MIC value against to the most bacteria while the others showed 4 mg L^–1. <br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>.