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A rapidly developing method to address global pollution issues is bioremediation. We designed a microbial biosensor based on Pseudomonas aeruginosa for the bioremediation approach for the removal of phenol from an aqueous solution. This bacterium was highly effective in using phenol as a carbon source and maximum degradation of phenol (750 ppm) was achieved using 10% (v/v) of 18 h old inoculum at pH 7 and 35°C in 135 h.