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<em>Lactobacillus</em> <em>acidophilus</em> bacterium is one of the most useful specieses of <em>lactobacillus</em> genus, which can be seen as a probiotic microorganism in dairy products like yoghourt, milk and some portions of mammal digestive systems like human intestine. The bacterium is positive gram and negative catalaze, and has the best growth in 37 °C during 72 hours in microaerophil condition. In this research, local strains of <em>Lactobacillus</em> <em>acidophilus</em> are dissected from 20 yoghourt samples of Shahr babak city of kerman. In order to identify this bacterium from non-molecular methods including growth study in different degrees, studying of sensitivity or resistance to antibiotic disks and surveying growth behavior regarding to carbohydrate fermentation, As well as molecular methods partial 16S rRNA gene sequencing were used. Using these examinations, 12 isolates of <em>Lactobacillus</em> <em>acidophilus</em> were extracted, where coincidanced with the positive samples noted in the bergey´s manual of systematic bacteriology. This is the first state report for isolation of <em>Lactobacillus</em> <em>acidophilus</em> isolates for local yoghourt of Shahr babak city of kerman.