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Little is known about the pharmacological activity of <i>Monarda fistulosa</i> L. essential oils. To address this issue, we isolated essential oils from the flowers and leaves of <i>M. fistulosa</i> and analyzed their chemical composition. We also analyzed the pharmacological effects of <i>M. fistulosa</i> essential oils on transient receptor potential (TRP) channel activity, as these channels are known targets of various essential oil constituents. Flower (MEO_Fl) and leaf (MEO_Lv) essential oils were comprised mainly of monoterpenes (43.1% and 21.1%) and oxygenated monoterpenes (54.8% and 77.7%), respectively, with a high abundance of monoterpene hydrocarbons, including <i>p</i>-cymene, γ-terpinene, α-terpinene, and α-thujene. Major oxygenated monoterpenes of MEO_Fl and MEO_Lv included carvacrol and thymol. Both MEO_Fl and MEO_Lv stimulated a transient increase in intracellular free Ca²⁺ concentration ([Ca²⁺]_i) in TRPA1 but not in TRPV1 or TRPV4-transfected cells, with MEO_Lv being much more effective than MEO_Fl. Furthermore, the pure monoterpenes carvacrol, thymol, and β-myrcene activated TRPA1 but not the TRPV1 or TRPV4 channels, suggesting that these compounds represented the TRPA1-activating components of <i>M. fistulosa</i> essential oils. The transient increase in [Ca²⁺]_i induced by MEO_Fl/MEO_Lv, carvacrol, β-myrcene, and thymol in TRPA1-transfected cells was blocked by a selective TRPA1 antagonist, HC-030031. Although carvacrol and thymol have been reported previously to activate the TRPA1 channels, this is the first report to show that β-myrcene is also a TRPA1 channel agonist. Finally, molecular modeling studies showed a substantial similarity between the docking poses of carvacrol, thymol, and β-myrcene in the binding site of human TRPA1. Thus, our results provide a cellular and molecular basis to explain at least part of the therapeutic properties of these essential oils, laying the foundation for prospective pharmacological studies involving TRP ion channels.