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AIM:To investigate the application value of contrast-enhanced ultrasound(CEUS)and analysis software for intra-ocular tumor diagnosis.<p>METHODS:Retrospective analysis for 90 cases(90 eyes)with intra-ocular tumor with CEUS examination, and all cases were confirmed by pathology after surgery or clinical comprehensive diagnosis and follow-up. <p>RESULTS:Choroidal hemangioma, choroidal melanoma, choroidal metastatic carcinoma had obvious different CEUS enhancement pattern, quantitative analysis indexes of those tumors by imaging analysis software were significantly different(<i>P</i><0.05). <p>CONCLUSION:The CEUS can provide quantitative analysis for intra-ocular tumor with preferable clinical application value.