Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells
oleh: Santosh Adhikari, Joe Moscatelli, Elizabeth M. Smith, Chiranjib Banerjee, Elias M. Puchner
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-07-01 |
Deskripsi
Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lysosomes at single-molecule resolution.