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The study aimed to show the effect of diet in observing some immunological and physiological aspects of the human body. The study is divided into two parts; the first part includes the immunological markers that involve interleukins (IL1α, IL 17,) and high-sensitivity C-reactive protein (hs-CRP). The second part includes biochemical markers; Vitamin D, Total cholesterol, Triglyceride, HDL, LDL, and Calcium. These parameters were measured in blood from 120 volunteers and their age (34.5 ± 9. 13) for men, and (27.4 ± 7.74) for women. However, the current study included two groups: Group - A (Include men blood pre and post-diet), and Group- B (Include blood sample of women pre and post-diet). IL1α, IL 17, hs-CRP, and Vitamin D were measured by using Sandwich Enzyme-Linked Immune-Sorbent assay technique (ELISA) while Total cholesterol, Triglyceride, HDL, LDL, and calcium were estimated by using the enzymatic colorimetric method. The average weight for men were (pre-diet = 94.1 ± 7.74; post-diet = 79.1 ± 12.8) while for women were (pre-diet = 78.3 ± 13.7; post-diet = 66.1 ± 10.3). The study noted that there is a statistically significant difference between groups (p<0.05) for the following indicators: Vitamin D, Total cholesterol, and group B for calcium assay. The study revealed that the results were non-significant between groups for parameters: IL1α, IL17, hs-CRP, Tg, HDL, and group A for calcium assay.