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The aim of this study was to determine the effects of various NH₄⁺/NO₃⁻ ratios in a nutrient solution on the growth and nitrogen uptake of Chinese kale under hydroponic conditions. The four NH₄⁺/NO₃⁻ ratios in the nutrient solution were CK (0/100), T1 (10/90), T2 (25/75), and T3 (50/50). An appropriate NH₄⁺/NO₃⁻ ratio (10/90, 25/75) promoted the growth of Chinese kale. T2 produced the highest fresh and dry weight among treatments, and all indices of seedling root growth were the highest under T2. A high NH₄⁺/NO₃⁻ ratio (50/50) promoted the growth of Chinese kale seedlings at the early stage but inhibited growth at the late growth stage. At harvest, the nutrient solution showed acidity. The pH value was the lowest in T3, whereas NH₄⁺ and NH₄⁺/NO₃⁻ ratios were the highest, which caused ammonium toxicity. Total N accumulation and N use efficiency were the highest in T2, and total N accumulation was the lowest in T3. Principal component analysis showed that T2 considerably promoted growth and N absorption of Chinese kale, whereas T3 had a remarkable effect on the pH value. These findings suggest that an appropriate increase in NH₄⁺ promotes the growth and nutrient uptake of Chinese kale by maintaining the pH value and NH₄⁺/NO₃⁻ ratios of the nutrient solution, whereas excessive addition of NH₄⁺ may induce rhizosphere acidification and ammonia toxicity, inhibiting plant growth.