No substantial preexisting B cell immunity against SARS-CoV-2 in healthy adults
oleh: Meryem Seda Ercanoglu, Lutz Gieselmann, Sabrina Dähling, Nareshkumar Poopalasingam, Susanne Detmer, Manuel Koch, Michael Korenkov, Sandro Halwe, Michael Klüver, Veronica Di Cristanziano, Hanna Janicki, Maike Schlotz, Johanna Worczinski, Birgit Gathof, Henning Gruell, Matthias Zehner, Stephan Becker, Kanika Vanshylla, Christoph Kreer, Florian Klein
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2022-03-01 |
Deskripsi
Summary: Preexisting immunity against SARS-CoV-2 may have critical implications for our understanding of COVID-19 susceptibility and severity. The presence and clinical relevance of a preexisting B cell immunity remain to be fully elucidated. Here, we provide a detailed analysis of the B cell immunity to SARS-CoV-2 in unexposed individuals. To this end, we extensively investigated SARS-CoV-2 humoral immunity in 150 adults sampled pre-pandemically. Comprehensive screening of donor plasma and purified IgG samples for binding and neutralization in various functional assays revealed no substantial activity against SARS-CoV-2 but broad reactivity to endemic betacoronaviruses. Moreover, we analyzed antibody sequences of 8,174 putatively SARS-CoV-2-reactive B cells at a single cell level and generated and tested 158 monoclonal antibodies. None of these antibodies displayed relevant binding or neutralizing activity against SARS-CoV-2. Taken together, our results show no evidence of competent preexisting antibody and B cell immunity against SARS-CoV-2 in unexposed adults.