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Owing to the development of modern display technology, micro- and mini-light-emitting diodes (LEDs) have affected technological advancements in the display industry. The structure of mini-LEDs is completely different than that of traditional LED backlights because the former employs a display technology with an LED array structure, optical resonant cavity, and color conversion layer. Display backlight technology encounters difficulty in achieving a truly effective energy-loss mechanism when designing or measuring mini-LED performance. The present study proposes three major mini-LED energy loss mechanisms: cross-talk, resonant, and quantum conversion losses. Herein, the spectrum was obtained through the integrating sphere system, and the calculation mechanism was formed by the down-conversion theorem as the decoupling principle, which successfully calculated the independent energy-loss efficiency of each mechanism and effectively deduced the brightness and color saturation performance of this design combination.