Duck TRIM29 negatively regulates type I IFN production by targeting MAVS
oleh: Weiqiang Li, Weiqiang Li, Weiqiang Li, Yating Song, Yating Song, Yating Song, Yuqing Du, Yuqing Du, Yuqing Du, Zhanhong Huang, Zhanhong Huang, Zhanhong Huang, Meng Zhang, Meng Zhang, Meng Zhang, Zuxian Chen, Zuxian Chen, Zuxian Chen, Zhuoliang He, Zhuoliang He, Zhuoliang He, Yangbao Ding, Yangbao Ding, Yangbao Ding, Junsheng Zhang, Junsheng Zhang, Junsheng Zhang, Luxiang Zhao, Luxiang Zhao, Luxiang Zhao, Hailiang Sun, Hailiang Sun, Hailiang Sun, Peirong Jiao, Peirong Jiao, Peirong Jiao
| Format: | Article |
|---|---|
| Diterbitkan: | Frontiers Media S.A. 2023-01-01 |
Deskripsi
The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins. DuTRIM29 inhibited IFN-β and IRF7 promoter activation in a dose-dependent manner and downregulated the mRNA expression of IFN-β, IRF7, Mx and IL-6 mediated by duRIG-I. Moreover, duTRIM29 interacted and colocalized with duMAVS in the cytoplasm. DuTRIM29 interacted with duMAVS via its C-terminal domains. In addition, duTRIM29 inhibited IFN-β and IRF7 promoter activation and significantly downregulated IFN-β and immune-related gene expression mediated by duMAVS in ducks. Furthermore, duTRIM29 induced K29-linked polyubiquitination and degradation of duMAVS to suppress the expression of IFN-β. Overall, our results demonstrate that duTRIM29 negatively regulates type I IFN production by targeting duMAVS in ducks. This study will contribute to a better understanding of the molecular mechanism regulating the innate immune response by TRIM proteins in ducks.