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The complete chloroplast (cp) genome sequence of Tugarinovia mongolica Iljin has been characterized in this study. The cp genome is 153,475 bp in length, containing a large single-copy (LSC) region of 84,434 bp and a small single-copy (SSC) region of 18,715 bp, which were separated by a pair of 25,163 bp inverted repeat regions (IRs). There are 114 unique genes annotated, including 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. The overall GC content is 37.62%. Further, phylogenetic analysis suggested that Tugarinovia is clustered to genus Atractylodes.