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Fermentative Production of Volatile Metabolites Using <i>Brettanomyces bruxellensis</i> from Fruit and Vegetable By-Products
oleh: Melodie A. Lindsay, Ninna Granucci, David R. Greenwood, Silas G. Villas-Boas
Format: | Article |
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Diterbitkan: | MDPI AG 2022-09-01 |
Deskripsi
Natural sources of flavour and aroma compounds are highly sought by the modern consumer; however, traditional sources are often low-yielding, and global supply is often outstripped by consumer demand. Fermentation is a favourable route by which natural flavours and fragrances can be produced. A non-<i>Saccharomyces</i> yeast, <i>Brettanomyces</i> <i>bruxellensis</i>, was investigated for its fermentative potential for the production of flavour and aroma metabolites from juice industry by-products: apple pomace, carrot pomace, and orange pomace. Submerged solid-substrate fermentations were carried out using sterile by-products without nutrient supplementation. Gas chromatography–mass spectrometry was used for volatile metabolite profiling of fermented substrates. One compound of interest, phenylethyl alcohol (rose fragrance), was extracted and quantified using GC-MS at a yield of 2.68 g/kg wet carrot pomace weight. This represents a novel, natural production strategy for phenylethyl alcohol compared to the traditional steam distillation of <i>Rosa domascus</i> sp. petals.