Tuning flavin environment to detect and control light-induced conformational switching in Drosophila cryptochrome
oleh: Siddarth Chandrasekaran, Connor M. Schneps, Robert Dunleavy, Changfan Lin, Cristina C. DeOliveira, Abir Ganguly, Brian R. Crane
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-02-01 |
Deskripsi
Chandrasekaran et al. engineered the Drosophila circadian clock protein Cryptochrome (dCRY) to form the neutral semiquinone, which serves as a dark-state proxy. They find that the C-terminal tail of dCRY remains docked when the flavin ring is reduced but uncharged. dCRY His378 variants provide insights into the recognition motifs for dCRY turnover and strategies for optogenetic tools.