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This study evaluated the effect of the extract and fractions of <i>Bauhinia holophylla</i> on <i>Candida albicans</i> planktonic growth, biofilm formation, mature biofilm, and hyphae growth. Three <i>C. albicans</i> strains (SC5314, ATCC 18804, and ATCC 10231) were tested. The crude extract and the fractions were obtained by exhaustive percolation and liquid–liquid partition, respectively. Phytochemical analyses of <i>B. holophylla</i> extract and fractions were performed using high-performance liquid chromatography coupled with a diode-array detector and mass spectrometry (HPLC-DAD-MS). A microdilution assay was used to evaluate the effect of the <i>B. holophylla</i> extract and fractions on <i>C. albicans</i> planktonic growth, and crystal violet staining was used to measure the total biomass of the biofilm. Hyphae growth was analyzed using light microscopy. Thirteen flavonoids were identified, with a predominance of the flavonol-3-<i>O</i>-glycoside type based on quercetin, myricetin, and kaempferol. Flavonoid-rich fractions of <i>B. holophylla</i> leaves displayed antifungal activity and inhibited both biofilm formation and hyphae growth in all the tested strains, but were not effective on <i>C. albicans</i> planktonic growth and mature biofilm. This study indicates that flavonoid-rich fractions from <i>B. holophylla</i> leaves interfere with the virulence of <i>Candida</i> species and support the use of <i>Bauhinia</i> spp. in folk medicine to treat infections.