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Background: Leukemia is one cause of death worldwide. Nowadays, natural therapies such as the use of medicinal plants may reduce adverse side effects of leukemia. Accordingly, the anticancer properties of medicinal plants have been recognized for centuries. This study focuses on Thai flowers from traditional drug recipes including phikun (Mimusops elengi Linn.), boonark (Mesua ferrea Linn.), ketawa (Gardenia jasminoides J.Ellis), and sarapi (Mammea siamensis (Miq.) T. Anders) for cytotoxicity and leukemia suppression. Fms-like tyrosine kinase 3 (FLT3) is a prognostic marker for acute myeloblastic leukemia (AML) and leukemic cell proliferation. Thus, FLT3 is a reliable marker for minimal residual disease (MRD) assessment in leukemia patients. Objectives: To investigate the cytotoxicity of crude ethanolic extracts from four Thai flowers and to determine their effects on FLT3 protein expression in EoL-1 cells. Materials and methods: Phikun, boonark, ketawa, and sarapi flowers were extracted by using 95% ethanol. An MTT assay was performed to evaluate cytotoxicity of each crude ethanolic flower extract. The effective crude ethanolic flower extract was further determined its inhibitory effect on FLT3 protein expression by Western blot analysis. Total cell number was determined by the trypan blue exclusion method. Results: Crude ethanolic flower extracts from boonark demonstrated the strongest cytotoxic activity with the inhibitory concentration at 50% (IC50) values of 62.5±3.9 ug/mL. Moreover, it could decrease the total cell number and FLT3 protein expression by a time- and dose-dependent manner. Conclusion: The crude ethanolic flower extracts from boonark inhibited cell proliferation via the suppression of FLT3 expression. It could be suggested that crude ethanolic flower extract from boonark flower is a promising approach for new anti-leukemic drug candidates.