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Microwave thermotherapy (MT) is an oncological treatment. At presentthe invasive thermometer probes are clinically used for temperaturemeasuring during an MT. Any invasive handling of tumors is ofhigh-risk. A new possible method of noninvasive monitoring oftemperature distribution in tissue has been developed. An MT treatmentof the experimentally induced pedicle-tumors of the rat was prepared.For 100 rat samples a strong correlation between the mean gray level inthe ROIs in the ultrasound pictures and the invasively measuredtemperature in the range 37-44 °C was found. The correlationcoefficient of the mean gray level and the invasively measuredtemperature is 0.96a0.05. A system for representation of changes ofspatial temperature distribution of the whole tumor during MT ispresented.